ProPlate® multi-wells integrate microscope slide based array technology with automated microtiter plate processing. These products allow users to isolate reagents on virtually any microscope slide surface, quickly and easily, forming up to 64 leak-proof, wells per slide. All ProPlate slide chambers feature 7.5mm depth to provide a generous surface to volume ratio to facilitate mixing and washing steps. Measured drawings illustrate the variety of well sizes and spacing available to fit standard 1 x 25 x 75mm microscope slides. Designs feature center to center spacing for use with multichannel pipetting. Slides may be process individually or multiple slides may be quickly inserted into a frame having the standard footprint of a microtiter plate for use with liquid handing robotics or automated plate washers. To prevent evaporation during incubation steps, wells may be sealed by means of flexible seals strips or lidded frames. After processing, slides may be quickly disassembled from the ProPlate and read using conventional chip readers. ProPlate materials may be washed and re-used multiple times. ProPlates are compatible with Grace’s ONCYTE ® Film-slide products for protein microarrays and most of the reagents common to use with microarray assays.
ProPlate® multi-wells are comprised of a rigid polycarbonate upper-structure and a compressible silicone gasket. A thin intervening layer of double-sided adhesive between the upper-structure and gasket serve to hold the gasket in position with respect to the alignment of the wells in both components. The adhesive layer does not come into contact with the reagents or sample substrate. Forming a seal between the silicone gasket and the microscope slide is achieved by applying either plastic snap-clips molded from delrin or stainless steel spring clips to the sandwiched assembly as shown in the illustrated instructions. With the exception of the snap-clips, all of the components of the system may be autoclaved and re-used multiple times. Standard chemical reference data for silicone and polycarbonate may be used to identify most chemical compatibilities with respect to reagents.
The isolation of multiple arrays on a single microscope slide is particularly well suited for applications such as protein expression analysis, protein-protein interactions; antibody profiling and HTP automated processing of multiple samples on replicate arrays. ProPlate® are used to process cDNA and oligonucleotide arrays and are even used for cell culture applications.
Q: We would like to use the ProPlate (16 well module) system for culturing cells. Can we use ProPlate for this application, and how are they best sterilized?
A: You can autoclave the ProPlate materials, after first removing the thin adhesive layer between the silicone and the polycarbonate superstructure. Do not autoclave the delrin snap clips. Stainless steel spring clips may be autoclaved. After careful assembly in the laminar flow hood, ProPlate modules are inserted into culture dishes, lidded and placed into a CO2 incubator. I have done this many times, my media usually contains Pen/Strep.