ProPlate® MTP features a biocompatible, pressure sensitive, adhesive bonding system applied to a bottomless microtiter plate to form a leak-proof seal between wells to isolate microarrays printed on glass or plastic during processing. Designs are available in black polystyrene for the 96 well (round) and 384 well (square) plates, and in black polycarbonate for the 96 well (square) plate, which maximizes the array printing area. Application of the substrate is as simple as peeling off a liner to expose the adhesive and pressing the content to the surface. Alignment of arrays within the wells may be facilitated by means of a fixture (sold separately). This product is often customized for content providers seeking a go-to-market solution for their arrays printed on difficult to bond substrates. Lids and adhesive plate seals are also available both as a packaging solution to protect content and as a means to prevent evaporation during reagent incubation steps. ProPlate MTP products are compatible with several available microarray imagers and most robotic liquid handling platforms.
ProPlate® MTP’s are fabricated from injection molded, bottomless polystyrene and polycarbonate plates with an ANSI-SLAS compliant footprint, height, well size and well spacing. Flatness across the bottom of the plate facilitates uniform “in focus” scanning or imaging of processed content. The “bottom” of each plate is coated with a compressible, biocompatible pressure sensitive adhesive with openings corresponding the size and shape of the wells. One surface of the adhesive is permanently bonded to the plate and the other surface of the adhesive has a removable liner which peels off easily, to expose the bonding surface. Using a simple, re-usable alignment fixture, a glass or plastic substrate measuring 74mm x 110mm may be aligned to the wells and pressed to form a leak-proof seal. Custom alignment fixtures for “over-sized” substrates are available at no extra charge. A device to apply and affix large quantities of plates is also available.
Peel the Liner
Apply the Glass
To quote Nielsen, et. al, from their publication in PNAS 2003 entitled Profiling receptor tryrosine kinase activation by using Ab microarrays “ High-throughput biological analysis requires efficient handling of multiple samples. This is accomplished most effectively by using 96- and 384-well microtiter plates.” This publication describes at length the methods used to demonstrate signal transduction in mammalian cells using a pre-printed Ab microarray affixed to the bottomless 96-well Proplate MTP. While microarray printers are available with the capability to print into the bottom of well plates, significant efficiency is gained by printing onto the substrate prior to application to the microwell plate. Employing the ProPlate MTP allows users to expand their use of commercially available surface treatments applied to the substrate that bind the array content.
Landry, J. P., G. Malovichko, and X. D. Zhu. “High-Throughput Dose–Response Measurement Using a Label-Free Microarray-in-Microplate Assay Platform.” Analytical Chemistry 87, no. 11 (June 2, 2015): 5640–48. doi:10.1021/acs.analchem.5b00720.
Nishizawa, Kazuki, Madoka Takai, and Kazuhiko Ishihara. “A Bioconjugated Phospholipid Polymer Biointerface with Nanometer-Scaled Structure for Highly Sensitive Immunoassays.” In Bioconjugation Protocols, edited by Sonny S. Mark, 491–502. Methods in Molecular Biology 751. Humana Press, 2011. http://dx.doi.org/10.1007/978-1-61779-151-2_31.
Nielsen, Ulrik B., Mike H. Cardone, Anthony J. Sinskey, Gavin MacBeath, and Peter K. Sorger. “Profiling Receptor Tyrosine Kinase Activation by Using Ab Microarrays.” Proceedings of the National Academy of Sciences 100, no. 16 (August 5, 2003): 9330–35.
Links to ANSI-SLAS Standards: http://www.slas.org/resources/information/industry-standards/