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PO Box 238, Bend, OR 97709

Phone: 541-318-1208
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Nitrocellulose Film Slides

2 slides on spot

Nitrocellulose-coated microarray substrates for protein, glycan and cell lysate arrays, tissue and cell printing and other applications where binding affinity, binding capacity and preservation of native bioactivities are important.

NEW! Oncyte SuperNOVA Thin Film Slides,  high binding capacity with low fluorescence background. More info here

Film Slide Selection Guide:

Now Here! PATH Nitrocellulose Thin Film Slides PDF file       More  information here

Oncyte Guide to Protein ArraysOncyte Guide to Protein Arrays 072011

ONCYTE® Nitrocellulose Film Slides and Film Plates

The nitrocellulose Film-slide and Film-plate technology, trademarked ONCYTE®, was invented by Grace Bio-Labs scientists .  The ONCYTE® product is a three dimensional microporous film cast on a variety of solid surfaces (vis. Glass, plastic) comprised of a combination of nitrocellulose polymer and proprietary chemistry that provides an ideal surface for marker discovery and validation as well as study of protein function.  ONCYTE® nitrocellulose Films excel in various applications including:    

  • Antibody/Protein Capture Microarrays  (Knezevic et al, 2001; Scorilas et al, 2000)
  • Cell or Tissue Lysate Microarrays  (Paweletz et al, 2001; Sheehan et al, 2005)
  • Lectin/Glycan Capture Microarrays  (Fukui et al, 2002; Wang D et al, 2002)
  • Whole Proteome Microarrays  (Davies et al, 2005a; Michaud et al, 2003)
  • Protein-Protein Interactions  (Espejo et al, 2002; Schweitzer et al, 2003)
  • Protein-DNA Interactions (Kersten et al, 2004; Ho et al, 2006)
  • Protein Domain Microarrays  (Espejo et al, 2002; MacBeath et al, 2002)
  • Cytocoherent Transfer  (McGrath  et al, 1991)
  • Peptide Microarrays  (Michaud et al, 2003; Poetz et al, 2005)
  • Tissue Printing  (Van Herpen et al, 2008; Wiley et al, 2007)

The performance advantages of ONCYTE® Film for each of these applications is crafted from chemistry manipulations.  Grace has built a strong set of Film Values with ONCYTE® 

  • Many stock Film patterns available on glass or plastic microscope slides--see below 
  • Film chemistry is resistant to standard Reblot™ and antigen retrieval protocols.
  • Coating is uniformly flat and amenable to automated printing with contact or non-contact devices
  • Can be coated on various surface chemistries (vis. Gold, Tantalum) 
  • Can be customized to any Film pattern
  • Strong R&D support for new applications
  • Strong quality support for variance control of key performance indicators

Typical autofluorescence backgrounds and binding capacities for Avid
Background Fluorescence.jpg Protein Binding Nova Avid.jpg
 
ONCYTE® offers uniformly low CVs with significant dynamic range:

AVID-Protein-Binding-Uniformity.gif

Protein-Binding-Capacity-Across-Production-Lots.gif

Intra-Slide-Variability.gif

 

AVID-Dynamic-Range.gif

 

 

Nitrocellulose Film Structure Provides Additional Assay Flexibility For Custom Applications

The parameters of nitration, pore size, pore density, pore shape and void volume all influence protein capture and detection sensitivities and can be systematically varied to meet specific demands.  For example, pore structure can be changed from an “open weave”, which is preferred for “flow through” applications, to a “closed pore” which is more suitable for high resolution microscopic applications (McGrath et al, 1991).

Similarly, void volumes can be controlled to accommodate chromatographic or other transfer applications.  Other chemistries can be added to Film; for example, chemistries to preserve phosphoproteins (Espina et al, 2008; 2009) or to lower autofluorescence backgrounds.  Nitrocellulose can be derivatized to put functional groups on the backbone (Wang X et al, 2009) to augment or change molecular binding properties (Wang X et al, 2010).




Download a presentation given at the 2011 American Association of Cancer Research Conference:  Microporous Nitrocellulose Surfaces For Increased Sensitivity and Dynamic Range of Protein Microarrays

 

 

pdficon_large.gif    Download Product Information

Other Products to use with ONCYTE®  Nitrocellulose-Coated Slides:

 

 Reblot™ is a trademark of Millipore, Inc.

™ and Nova™ Films are illustrated in the following graphs:


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