Reverse phase protein arrays (RPPA) offer great promise for the identification and analysis of critical biomarkers for disease states in patient samples and for high-throughput drug screening using cultured cell systems.  Advancing  RPPA technology into clinical diagnostics will require more consistency in methods  to allow for cross-study comparisons, obtain consensus results and ultimately, derive consistent and reliable patient diagnosis. Grace Bio-Labs has refined the production of premium nitrocellulose film slides which have the highest protein binding capacity and lowest fluorescent backgrounds.  We strive to provide a more comprehensive solution for RPPA use and accelerate its progression into the clinic by complementing optimized nitrocellulose film slides with lot-controlled reagents optimized for RPPA use.

Recent Events:

First Global RPPA Workshop at MD Anderson, October, 2011.

Held at MD Anderson Cancer Center October 10-11, the first Reverse Phase Protein Array Global Workshop brought together over 100 scientists from around the world to discuss standards for generating and analyziing results using Reverse Phase Protein Arrays. The following poster was presented from Grace Bio-Labs and discusses our development efforts to provide optimal substrate and reagents for RPPA technology.

Recent Publications in RPPA:

High resolution mapping of the cardiac transmural proteome using reverse phase protein microarrays. Mol. Cell Proteomics 10(7)M111, 2011.  Anderson, T., Wulfkuhle, J.,m Petricoin, E.3rd, Winslow, R.L.

Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous. Mol. Vis. 17:2856, 2011. Ecker SM, Hines JC, Pfahler SM, Glaser BM

Adhesion molecule protein signature in ovarian cancer effusions is prognostic of patient outcome. Cancer Aug 25, 2011. Kim G, Davidson B, Henning R, Wang J, Yu M Annunziata C, Hetland T, Kohn EC.

J Lipid Res. 2011 Dec;52(12):2323-31. Epub 2011 Oct 4.

Multidimensional profiling of plasma lipoproteins by size exclusion chromatography followed by reverse-phase protein arrays. J. Lipid Res. 52(12): 2323, 2011. Dernick G., Obermuller S., Mangold C., Magg C., Matile H., Gutmann O., von der Mark E., Handschin C., Maugeais C., Niesor EJ.

DATA SHEET: Super G Blocking Buffer mimizes fluorescence background.

Super G Blocking buffer is under development at Grace Bio Labs. Designed to minimize fluorescence background for nitrocellulose-based protein arrays.