Employs the quantitative sandwich enzyme linked immunosorbent assay (ELISA) technique to identify or select specific cell immunophenotypes.

Either a monoclonal or polyclonal antibody specific for chosen analyte may be pre-coated onto membrane coated microscope slides.

Appropriately stimulated cells are pipetted into the wells and the slide is placed into a humidified 37°C CO2 incubator for a specified period of time.

During this incubation period, the immobilized antibody in the immediate vicinity of the secreting cells, binds secreted analyte.  After washing away any cells and unbound substances, a biotinylated antibody specific for the chosen analyte is added to the wells.  Following a wash to remove unbound biotinylated antibody, alkaline-phosphatase conjugated to streptavidin or fluorescent streptavidin is added.  Each individual color or flourescent spot represents an individual analyte-secreting cell.  The spots can be counted with a reader system or manually, using a stereomicroscope.

Uses Standard 25mm x 75mm Slides.

Eliminates use of leaky, membrane bottom microtitre plate assay. 

Reduces sample number to cost effective reagent quantities, and sample size. 

Eliminates use of vacuum or centrifuge washes.

Film coating: 0.45um PVDF allows maximum protein binding for flourescent & colorimetric detection.
Removable chamber for scanning & reading.
 
Chamber housing removes cleanly & easily.
 
Cell Phenotyping assay may be developed by the application of protein arrays on films and selective binding of cells.